Figure 4: TAT-SALL4B was transcriptionally active in vivo. (A) Schematic diagram of experimental procedures. (B) After HEK293T cells were transfected with OCT4-driven luciferase and Renilla-luciferase expression plasmids, cells were cultured in the medium containing different concentrations (5, 10, 50, 100 nM) of purified recombinant TAT-SALL4B for 48 h. Control groups were supplemented with either BSA or various concentrations of heat-inactivated SALL4B (50, 100 nM). After incubation, cell lysates were prepared and assayed for firefly and renilla luciferase activities. Firefly luciferase activity was normalized to renilla luciferase activity and the activity of the control group supplemented with BSA was set as 1.0. Bars represent the mean of 3 replicates with standard derivation. The symbol ** means P < 0.01 and * means P < 0.05 as determined by two-tailed Student’s t-test. The data were representative of two independent experiments.