Figure 5: A and B: Changes in cell size of immortalized HL1-hT1 cells. Cells were cultured in the proliferation medium without and with different concentrations of alcohol for 4 days. Values are mean ± SEM. N = 4 sets of cell cultures in each group. Bars with different letters in panel B are statistically different (p<0.05). C: Changes in cyclin D1 expression by immortalized HL1- hT1 cells. Cells were cultured in the proliferation medium without and with different concentrations of alcohol for 24 h. Values are mean ± SEM. N = 5 sets of cell cultures. FSC=forward side scatter. Bars with different letters are statistically different (p<0.05).