Figure 4: Immunocytochemistry. Immunolocalization of both surface antigens and pluripotency associated markers in bWJ-MSCs monolayer. Cells were stained with primary antibodies directed against (A) CD73, (D) CD90, (G) CD105, (J) Oct4, (M) Sox2, and (P) Nanog, (S) CD34 amd stained with FITC conjugated secondary antibodies. In panels (B, E, H, K, N, Q, U) representative fields as observed under bright field. In panels (C, F.I, L, O, R, T) flurescent blue coloured DAPI stained representative fields.