| Target genes |
Primer sequences, 5′-3′ |
Gene bank accession no |
Annealing temperature |
Product size (kb) |
| Oct4 |
S: GAGCCGAACCCTGAGGAG
A: AGGGTAAGCCCCACATCG |
JF898834.1 |
60 |
125 |
| Sox |
S: CTATGACCAGCTCGCAGAC
A: ACTTCACCACCGAGCCCA |
GQ451841.1 |
60 |
111 |
| Nanog |
S: GCAGGTGAA GACCTG GTTC
A: CCACATGGGCAGGTT TCCA |
JF 898835.1 |
60 |
175 |
| β actin |
S: AGA TTG GCA TGG CTT TAT TTG T
A: CTG TAG AAC TTT GGG AAT GCT C |
BT030480 |
51 |
143 |
|
