Figure 2: Expression of ES cell pluripotent pluripotency markers in piPS/ hrGFP+ cells. (A) Immunocytochemistry of piPS/hrGFP+ cells with antibodies to Oct4 (A), AP (B), SSEA-3 (C), SSEA-4 (D), TRA-1-60 (E), and TRA-1-81 (F). Nuclei were stained with DAPI (blue). (B) RT-PCR analysis of ES cell pluripotency genespluripotent marker genes (G and H).