| Gene |
Sequences |
Length (bp) |
Annealing (°C) |
| pOct4 |
F: 5'-AGGTGTTCAGCCAAACGACC-3'
R: 5'-TGATCGTTTGCCCTTCTGGC-3' |
335 |
60 |
| pSox2 |
F: 5'-GCAACTCTACTGCTGCGGCG-3'
R: 5'-GCCATGCTGTTGCCTCC-3' |
352 |
60 |
| pKlf4 |
F: 5'-GCGGAGGAACTGCTAAG-3'
R: 5'-GCACTTCTGGCACTGGA-3' |
423 |
60 |
| pc-Myc |
F: 5'-TCGGACTCTCTGCTCTCCTC-3'
R: 5'-CTGCATAATTGTGCTGGTGC-3' |
274 |
60 |
| Actin |
F: 5'-CTCCTTAATGTCACGCACGATTTC-3'
R: 5'-GTGGGGCGCCCCAGGCACCA-3' |
539 |
60 |
