Figure 2: Characterization of hematopoietic stem cells isolated from C57BL/6 mice. The phenotype of cells from lung (A) and bone marrow (B) was studied on the protocol for hematopoietic stem cells (BD Biosciences). The HSC population taken through a Lin- selection (not shown), is shown gated Sca1+ and c-kit+ (P6), then displayed for CD34- (P7). The Lin-Sca1+c-kit+CD34- cells are long-term (LT) HSC, and the Lin-Sca1+c-kit+CD34+ cells are considered short-term (ST) HSC (not shown). Thus, all two of these populations can be readily sorted from one sample. (A1, B1) HSCs isolated from mice after intratracheal administration of NaCl 0.9% (7th day of the experiment); (A2, B2) HSCs isolated from mice after intratracheal bleomycin administration (7th day of the experiment); (A3, B3) HSCs isolated from mice after intratracheal bleomycin administration and treated spiperone (7th day of the experiment). Dot plots are representative figures of three independent experiments with the mean from three independent experiments.