Figure 4: Assay of cell-surface antigens on murine mesenchymal stem cells from lung (A) and bone marrow (B). Lung-derived MSCs (A) and BM-derived MSCs (B) were labeled with antibodies against CD31, CD34, CD44, CD45, CD73, CD90, CD106 antigens and analyzed by flow cytometry. Flow cytometry results from mice after intratracheal administration of NaCl 0.9% (A1, B1), mice after intratracheal bleomycin administration (A2, B2), mice after intratracheal bleomycin administration and spiperone-treated (A3, B3) on 21st day of the experiment. Our data imply that on the 21st day of the experiment lung and bone marrow CD45- cells of mentioned above groups mice expressed on the surface of CD44, CD77, CD90, CD106 and were negative for CD31 and CD34. The all of these populations is sorted from one sample. Dot plots are representative figures of three independent experiments with the mean percentage of at least three experiments.