Figure 8: Multilineage differentiation of lung-derived MSCs. Osteogenic differentiation. During osteogenic differentiation cells altered their shape and assembled in clusters. The cluster formation is considered a typical feature of osteogenic MSC differentiation in vitro. Osteogenic differentiation was demonstrated by the presence of mineralized nodules stained black/ purple with von Kossa staining. β-glycerophosphate significantly increased formation of mineralized nodules. Results are presented as mean and SEM. On the ordinate axis – area of mineralized nodules (mm2), on the abscissa - examined groups: mice with intratracheal administration of NaCl 0.9%, mice with intratracheal bleomycin administration, mice with intratracheal bleomycin administration and treated with spiperone.