| Groups (n – number of mice in group) |
Total mononuclear cells |
Neutrophils |
Macrophages |
Lymphocytes |
| Mice receiving IT NaCl 0.9% (n=10) |
271 ± 24 |
9 ± 1 |
16 ± 2 |
38 ± 2 |
| 3rd day after bleomycin instillation |
| Mice with fibrosis NaCl 0.9% treated (n=10) |
489 ± 46 * |
18 ± 2 |
29 ± 2 * |
81 ± 8 * |
| Mice with fibrosis spiperone treated (n=10) |
382 ± 31 * |
11 ± 1 & |
15 ± 1 & |
41 ± 3 & |
| 7th day after bleomycin instillation |
| Mice with fibrosis NaCl 0.9 % treated (n=10) |
457 ± 35 * |
25 ± 2 * |
38 ± 1 * |
98 ± 7 * |
| Mice with fibrosis spiperone treated (n=10) |
324 ± 29 & |
12 ± 1 & |
13 ± 1 & |
42 ± 4 & |
| 14th day after bleomycin instillation |
| Mice with fibrosis NaCl 0.9% treated (n=10) |
642 ± 59 * |
20 ± 2 * |
32 ± 2 * |
82 ± 9 * |
| Mice with fibrosis spiperone treated (n=10) |
425 ± 41 * & |
11 ± 1 & |
14 ± 1 & |
45 ± 4 & |
Lung tissues were obtained from C57BL/6 mice at the indicated time intervals after bleomycin treatment. The numbers of cell were counted per microscopic field at ×400magnification. Each value represents mean ± SEM. Data represent mean of 2 independent experiments. * P <0.05 - compared to the mice received intratracheal NaCl 0.9%;& P<0.05 - compared to the mice received intratracheal bleomycin and NaCl 0.9% treated.