Day after bleomycin instillation Mice received intratrachealNaCl 0.9% Mice with fibrosis NaCl 0.9% treated Mice with fibrosis Spiperone treated
7 1.12 ± 0,05 3.07 ± 0.07 * 2.09 ± 0.11 &
14 1.13 ± 0,02 3.91 ± 0.07 * 2.75 ± 0.12 *&
21 1.15 ± 0,07 4.26 ± 0.14 * 2.12 ± 0.13 *&
The content of collagen fibers in lung was determined using a function for counting the area of the object in the image. Broncho-vascular strands were carefully removed from the analyzed areas. The relative fibrotic tissue area is calculated of by the formula: X = Σa x 100/(S - Σb)
where: a - is the amount of pixels occupied by fibrotic tissue in 10 pictures of one drug, S - is the number of pixels corresponding to the total area of the image (using this camera and software - 4,423,680), b - a sum of pixels occupied by the empty part of the glass slide in 10 pictures of one drug.
Data represent mean of 3 independent experiments, n = 10/group. Results are presented as mean and SEM. * P<0.05 - compared to the mice received intratracheal NaCl 0.9%; & P<0.05 - compared to the mice received intratracheal bleomycin and NaCl 0.9% treated.
Table 2: The content of the connective tissue in the lungs of C57BL/6 mice (% of the area of lung tissue).