| Groups(n – number of mice in group) |
Collagen type I (ng/right lung lobe) |
Hydroxyproline (ng/right lung lobe) |
Total soluble collagen (μg/right lung lobe) |
| Mice received intratrachealNaCl 0.9% (n=10) |
121.1 ± 11.7 |
2915 ± 240 |
69.6 ± 6.3 |
| Mice with fibrosis NaCl 0.9% treated (n=10) |
233.3 ± 22.4* |
8714 ± 651* |
127.2 ± 11.4* |
| Mice with fibrosis spiperone treated (n=10) |
156.8 ± 12.1& |
5693 ± 298& |
101.2 ± 6.1& |
Hydroxyproline, collagen type I and total soluble collagen levels were measured in homogenate of right lung lobes from C57BL/6 mice (М ± m). Data represent mean of 2 independent experiments, Hydroxyproline and collagen type I were determined by ELISA according to manufacturer instructions (Cusabio Biotech CO., Ltd, China). The total soluble collagen was determined using a standard curve for the SircolTM assay (Biocolor Ltd, UK) according to manufacturer’s instructions. Results were expressed as μg collagen per right lung. Data represent mean of 2 independent experiments. Results are presented as mean and SEM. * P<0.05 - compared to the mice received intratracheal NaCl 0.9%; & P<0.05 - compared to the mice received intratracheal bleomycin and NaCl 0.9% treated. |
