Figure 3: Ex-vivo kidney perfusion platform. Up to 48 hours post initial treatment with glomerulopathic LCs. RMCs interacting with damaged mesangium. Transmission electron microscopy, uranyl acetate and lead citrate stain. AX 7000; BX17500; X7000; DX14000; EX15500; FX17500. A and B- MSCs in glomerular capillary lumina (circle A). The stem cells are small, exhibit a large nuclear to cytoplasmic ratio and minimal amounts of cytoplasm with few organelles (circle B). MSCs identify and approach the damaged mesangial area, embracing it with cytoplasmic extensions (C) (circle) and eventually incorporate themselves into the mesangium (E, F circle) where they proceed to clear debris and eventually differentiate into mesangial cells acquiring more cytoplasm, intracytoplasmic myofilaments, and attachment plaques at their periphery (E).