Primer |
Sequence (5’-3’) |
Annealing |
Oct4 F
R |
AGCTGCTGAAGCAGAAGAGG
GGTTCTCATTGTTGTCGGCT |
61°C |
Oct-4A F
R |
AACCGTCCCTAGGTGAGCCG
CCCACCTGGAGGCCCTTGGAA |
63°C |
Sca-1 F
R |
AGAGGAAGTTTTATCTGTGCAGCCC
TCCACAATAACTGCTGCCTCCTGA |
66°C |
Gfra F
R |
GGCCTACTCGGGACTGATTGG
GGGAGGAGCAGCCATTGATTT |
58°C |
Prohibitin F
R |
GTGGCGTACAGGACATTGTG
AGCTCTCGCTGGGTAATCAA |
58°C |
Protamine F
R |
GGCCACCACCACCACAGACACAGGCG
TTAGTGATGGTGCCTCCTACATTTCC |
66°C |
Gapdh F
R |
GTCCCGTAGACAAAATGGTGA
TGCATTGCTGACAATCTTGAG |
58°C |
Dazl F
R |
GTGTGTCGAAGGGCTATGGAT
ACAGGCAGCTGATATCCAGTG |
61°C |