| Procedure |
GMP associated information for ASCs production |
Reference |
| Collection |
fat pad, liposuction aspiration or Lipogems product |
[1,3,4] |
| Isolation |
Collagenase digestion vs.nonenzymatic method |
[1,4,5] |
| Purification |
Adherent culture vs. lineage-negative sorting |
[1,3,5,8] |
| Expansion |
Freshly isolated cells vs. cultured cells |
[8-11] |
| Characterization |
Immunophenotype, Differentiation capacity, Colony formation, Transcriptome and Proteome |
[3-8] |
| Clinical application |
Diabetes, ischemic injury, liver injury, kidney injury, cardiac repair, retinopathy, inflammatory diseases, transplant tolerance, erectile dysfunction, nerve repair, anti-cancer therapy |
[1,2,13] |
|
