| Expansion step |
Days of culture |
Cell seeded |
Cell harvested |
Viability |
Doubling time (h) |
Number of doublings |
| P0 |
Flask
Fresh cells |
14 |
252 E+06
(12.6 ml Ficoll BM) |
9.8 E+06
(P1F) |
100% |
NA |
NA |
Quantum
Fresh cells |
14 |
25 ml
(whole BM) |
12.3 E+06
(P1Q) |
92% |
NA |
NA |
| P1 |
Flask
Fresh cells |
7 |
9.7 E+06
(P1F) |
76 E+06 (P2F) |
98% |
56 |
3.0 |
Quantum
Thawed cells |
7 |
8.2 E+06 (P1Q) |
154 E+06
(P2Q) |
97% |
40 |
4.2 |
| P2 |
Flask
Fresh cells |
7 |
70 E+06
(P2F) |
425 E+06
(P3F) |
90% |
64 |
2.6 |
| P2 |
Quantum
Thawed cells |
7 |
20 E+06 (P2Q) |
169 E+06
(P3Q-1) |
93% |
54 |
3.1 |
Quantum
Thawed cells |
7 |
20 E+06 (P2Q) |
172 E+06
(P3Q-2) |
89% |
54 |
3.1 |
Quantum
Thawed cells |
7 |
20 E+06 (P2Q) |
110 E+06
(P3Q-3) |
89% |
68 |
2.5 |
|
