Expansion step Days grown Cell seeded Cell harvested Viability Doubling time (h) Number of doublings P2 Fresh cells Flask 7 140 E+06 (P2F) 870 E+06 (P3F) 95 % 63 2.6 P2 Thawed cells Quantum 7 20 E+06 (P2FT) 276 E+06 (P3Q-1) 93 % 44 3.8 Flask 8 80 E+06 (P2FT) 209 E+06 (P3FT-1) 95 % 138 1.4 Quantum 7 20 E+06 (P2FT) 178 E+06 (P3Q-2) 97 % 53. 3.2 Flask  9 80 E+06 (P2FT) 249 E+06 (P3FT-2) 95 % 132 1.6 Quantum 7 20 E+06 (P2FT) 170 E+06 (P3Q-3) 78 % 54 3.1 Flask 8 80 E+06 (P2FT) 161 E+06 (P3FT-3) 95 % 190 1.0

Table 3: Results of P2 expansion from P2 thawed MSC in the Quantum® device or traditional flasks. Results were also compared with those obtained after P2 from fresh cells from the same donor (Figure 2).