Expansion step Days of culture Cell seeded Cell harvested Viability Doubling time (h) Number of doublings
Donor 1
Fresh cells
Flask 7 224 E+06 (P2F-1) 1092 E+06 (P3F-1) 84% 73 2.3
Donor 1
Thawed
cells
Quantum 7 50 E+06 (P2FT-1) 297 E+06 (P3Q-1A) 92% 65 2.6
Flask 8 50 E+06 (P2FT-1) 109 E+06 (P3FT-1A) 91% 170 1.1
Quantum 8 50 E+06 (P2FT-1) 291 E+06 (P3Q-1B) 95% 75 2.5
Flask  10 42 E+06 (P2FT-1) 74 E+06 (P3FT-1B) 95% 294 0.8
Donor 2
Fresh cells
Flask 7 172 E+06 (P2F-2) 560 E+06 (P3F-2) 82% 98 1.7
Donor 2
Thawed
cells
Quantum 8 50 E+06 (P2FT-2) 334 E+06 (P3Q-2) 94% 70 2.8
Table 5: Results for P2 expansions from P2 thawed MSC in the Quantum® device and traditional flasks. Results were also compared with those previously obtained after P2 from fresh cells from the two same donors (Figure 3).