Expansion step |
Days of culture |
Cell seeded |
Cell harvested |
Viability |
Doubling time (h) |
Number of doublings |
Donor 1
Fresh cells |
Flask |
7 |
224 E+06 (P2F-1) |
1092 E+06 (P3F-1) |
84% |
73 |
2.3 |
Donor 1
Thawed
cells |
Quantum |
7 |
50 E+06 (P2FT-1) |
297 E+06 (P3Q-1A) |
92% |
65 |
2.6 |
Flask |
8 |
50 E+06 (P2FT-1) |
109 E+06 (P3FT-1A) |
91% |
170 |
1.1 |
Quantum |
8 |
50 E+06 (P2FT-1) |
291 E+06 (P3Q-1B) |
95% |
75 |
2.5 |
Flask |
10 |
42 E+06 (P2FT-1) |
74 E+06 (P3FT-1B) |
95% |
294 |
0.8 |
Donor 2
Fresh cells |
Flask |
7 |
172 E+06 (P2F-2) |
560 E+06 (P3F-2) |
82% |
98 |
1.7 |
Donor 2
Thawed
cells |
Quantum |
8 |
50 E+06 (P2FT-2) |
334 E+06 (P3Q-2) |
94% |
70 |
2.8 |
|