300x10
6
harvestedcells
Quantum (thawed cells)
Flask (fresh cells)
Flask (thawed cells)
Loaded cells
50x106
62x106
138x106
Days in culture
7
7
8
Disposables (kits, bags, flasks)
1 disposable kit+bags
88 flasks
198 flasks
Medium consumption
7,840 mL
5,040 mL
11,200 mL
Working time / run (hours)
8
12
21
Working time/harvest+freezing (hours)
3
7
15
Table 7:
Quantum® device and T-flask MSC manufacturing requirements (P2 expansion step). Comparison between Quantum® and T-flask (fresh or thawed cells) manufacturing needs considering a P2 expansion step leading to a 300x10
6
MSC harvest.