Figure 4: Expression of (a) SP7, (b) ALP, (c) IBSP, and (d) SPP1 genes after MSCs were cultured in GM and mixture medium of OM and AM at a ratios of 100/0, 95/5, 85/15, 70/30, 20/80, and 0/100 for 21 days. The gene expression was investigated by real-time PCR analysis. Data represent mean ± SD (n=3, n represents the number of duplicate samples of cultured cells at the same condition). *, P< 0.05, **, P< 0.01, ***, P< 0.001.