Figure 1: GPC3 expression in HCC cells and its correlation with self-renewal. A. Expression profiling of liver CSC markers by flow cytometry in normal hepatocytes (NH), tumor associated fibroblasts (TAFs), and malignant hepatocytes isolated from HCC patients and HCC cell lines. B. Two-color flow cytometry analysis on the colocalization of cell surface expression of GPC3 with CSC markers EpCAM and CD133. C. Expression of GPC3, EpCAM, CD133, CD24, and CK-19 proteins in HCC cell lines with different tumorigenic potentials. D. Immunofluorescence staining of GPC3 (green) and DAPI (blue) showing cell surface location of GPC3 in HepG2 and Hep3B cells (Magnification=400X).