Figure 4: Regulation of cell cycle progression from G0/G1 to S phase by GPC3 via activation of autophagy. A. Cell cycle analysis of GPC3-high and GPC3-low cells isolated from Huh7 and Hep40 cells. B. Western blotting of cell cycle regulators in isolated GPC3-high and GPC3-low sub-populations from Huh7 and Hep40 cells. C. Protein expression of cell cycle regulators and cell cycle analysis of SNU-449 cells with un-induced (no-Dox), or induced (Dox) GPC3). D. Effects of experimental induction of starvation-induced autophagy and its inhibition by 3-MA (10 mM) on the protein levels of p21, p15INK4B and phosphorylation of Rb in SNU-449 cells with un-induced (no-Dox), or induced (Dox) GPC3. E. Effects of 3-MA on cell cycle progression in Huh7 and Hep40 cells as analysed by flow cytometry.