One step system (in line) (Cellex®) Two stepsystem (off line)
Main principle Integrated system: sequences of cell separation-UVA irradiation-infusion Mononuclear cell apheresis and separated UVA irradiation of the total harvested cells
UVA dose 1.2 J/cm2 2 J/cm2
Sample collection for quality control No Yes
Nb of Vascular access One Two
Total duration of course 1 h 30 min to 2 hours 3 to 4 hours
Pediatric use Yes but restricted to >40 kg Yes-no restriction
Other limitations Cost Cell therapy unit required for UVA irradiation
Table 1: Practical considerations of the two main photopheresis systems.