Figure 2: Linear graphs of serially passaging of discospheres in suspension culture in two experimental series. In the first experimental series, 300 spheres were collected from culture plates, on day 14 post plating, washed, prepared as single cell suspensions, and plated in suspension culture media and conditions, as above. The spheres from the second culture series were passaged twice more. Thus, a total of 3 passages were made from the stem cell culture product of the first culture series, demonstrated that linear expansion of the stem cell population was possible (Figure 2, black line graph). The black line graph represents the expansion of stem cells derived from the first culture series through serial passaging. In the second experimental series, several revisions, additions, and changes to the suspension culture protocols were made. Again, 300 spheres were collected from culture plates, on day 14 post plating, washed, prepared as single cell suspensions, and plated in suspension culture media and conditions, as above. The spheres from the second culture series were passaged twice more. Thus, a total of 3 passages were made from the stem cell culture product of the first culture series, and again demonstrated that linear expansion of the stem cell population was possible (Figure 2, red line graph).