Figure 2: Immunofluorescent staining and FISH. A: Immunofluorescent staining using Texas Red labeled antibody directed against CD3 detects T-cells (arrow heads) in the superficial dermis. Double immunofluorescent staining using the same antibody directed against human CD3 and a FITC labeled antibody against human CD20 does not show B-cells associated to T-cells (red arrow heads) in the superficial dermis. Bar=80 μm. B: XY fluorescent in situ Hybridization shows on the left picture numerous XX cells in the epidermis (E) of the female patient, and on the right picture, presence of XY cells around a vessel (V) in the dermis. Bar=80 μm.