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Figure 10 :Response to colony formation in presence of SCF-1 (5ng/ml) in
methycellulose containing IMDM A: Photomicrographs of colonies counted at day 7 of culture in defined 3D matrix. BJNhem20 in methyl cellulose with SCF-1 in IMDM- undifferentiated ES, EBs and differentiated in SAGM (BJNhem 19) (Photograph of colonies counted and data presented for Fig.10A, was taken using Bright field phase contrast microscope : model no Olympus BX41 ,camera model no Olympus DP20 at 40X magnification) B: CFU-c at day 7 of BJNhem19 in methyl cellulose with SCF-1 in IMDMundifferentiated ES, EBs and differentiated in SAGM (BJNhem 19) C: CFU-c at day 7 of BJNhem20 in methyl cellulose with SCF-1 in IMDMundifferentiated ES, EBs and differentiated in SAGM (BJNhem 20) |