Figure 1B: Confocal photomicrographs of Ad (G) cells. Ad (G) cells (panel a and b) were not stained by the anti-SMemb (panel c, diluted 1:1000) or anticalponin antibodies (panel d, diluted 1:100). Thus, Ad (G) cells expressed neither mature (calponin) nor immature (SMemb) SMC-specific proteins at this time. Magnification, x 100. Immunostaining of NIH3T3 fibroblast cells against the SMemb antibody and native smooth muscle cells against calponin antibody were shown as positive controls (panel e and f, respectively).