Figure 5: BM-derived SMCs showed a [Ca2+]i response to bradykinin. (A) Graph represents typical time course of [Ca2+]i in cells. Exposure to bradykinin induced a rapid increase in [Ca2+]i in BMSC-derived SMCs (red line), but not in bulk-stromal cells (black line). (B) Representative pseudocolor images, where red and yellow represent high and blue and black represent low [Ca2+]i concentrations. The pseudocolor images in fura-2-AM-loaded BMSC-derived SMCs (a and b) were acquired at the time corresponding to panels a and b in (A), respectively. As shown in lower panel, BM-derived SMCs showed the same [Ca2+]i response to angiotensin II.