Pluripotency stringency criteria |
Mouse model systems |
** In vitro morphology and gene expression |
** In vitro teratoma formation |
** In utero chimeric embryogenesis |
** Tetraploid aggregation and germline transmission |
Human model systems |
** In vitro morphology and gene expression |
** In vitro teratoma formation |
Identification properties of iPSCs |
Cellular biological properties |
Morphology, Growth, Telomere activity, Stem cell markers, Stem cell genes |
Pluripotency |
Neural differentiation, Cardiac differentiation, Teratoma formation, Embryoid body, Chemeric mice, Tetraploid complementation |
Epigenetic programming |
Promoter demethylation, DNA methylation globally, Histone demethylation |