Pluripotency stringency criteria
Mouse model systems ** In vitro morphology and gene expression
** In vitro teratoma formation
** In utero chimeric embryogenesis
** Tetraploid aggregation and germline transmission
Human model systems ** In vitro morphology and gene expression
** In vitro teratoma formation
Identification properties of iPSCs
Cellular biological properties Morphology, Growth, Telomere activity, Stem cell markers, Stem cell genes
Pluripotency Neural differentiation, Cardiac differentiation, Teratoma formation, Embryoid body, Chemeric mice, Tetraploid complementation
Epigenetic programming Promoter demethylation, DNA methylation globally, Histone demethylation
Table 2: Properties of iPSCs and their stringency criteria.