| Classes |
Types |
Examples |
DNA
Methods |
(1) Viral delivery systems: |
* Adenoviral transduction |
| * Retroviral transduction |
| * Lentiviral transduction |
| (2) Non-viral systems: |
** Episomal plasmids;Plasmid and episomal vectors |
(3) Conditional systemsand
transposons: |
* Cre -IoxP recombination |
| * Piggyback transposon |
Non-DNA
Methods |
(a) Protein - mediated: |
* Direct delivery of programmingproteins onto the cell |
| * Wnt 3a conditioned medium |
| (b) Manipulation of cellculture condition: |
- Lower amount of oxygen |
| (c) Use of histonedeacetylase inhibitors: |
** Valproic acid enhancesprogramming of iPSCs |
| (d) Sindae virus transduction |
|
| (e) Direct fusion withembryonic stem cell;cell fusion |
|
| (f) Extraction of embryonicstem cell; cell extract |
|
