Figure 4: Differentiation of Fabry-iPS cells into cells of a cardiac lineage and transduction of Fabry-iPS cells with lentiviral vectors (A) In vitro differentiation of Fabry-iPS cells into cardiac lineage. RT-PCR analysis of cardiac lineage-specific genes in contracting cells, OP9 stroma cells, and mouse embryonic stem cells (RF8). Gapdh was used as an internal control. (B) In vitro differentiation of Fabry-iPS cells into cells of a cardiac lineage. Real-time PCR analysis of cardiac lineage marker genes in Fabry-iPS-derived contracting cells and mouse ES cell (RF8)-derived contracting cells. Gapdh was used as an internal control. (C) Transduction of Fabry-iPS cells with a lentiviral vector (LV)/enGFP vector at a multiplicity of infection of 84. Transduced Fabry-iPS cells maintained SSEA-1 expression (upper right panel). (D) Intracellular α-galactosidase A (α-gal A) activity in LV/α-gal A-transduced Fabry-iPS cells. (E) Secreted α-gal A activity in LV/α-gal A-transduced Fabry-iPS cells. |