Figure 4: Differentiation of Fabry-iPS cells into cells of a cardiac lineage and transduction of Fabry-iPS cells with lentiviral vectors
(A) In vitro differentiation of Fabry-iPS cells into cardiac lineage. RT-PCR analysis of cardiac lineage-specific genes in contracting cells, OP9 stroma cells, and mouse
embryonic stem cells (RF8). Gapdh was used as an internal control.
(B) In vitro differentiation of Fabry-iPS cells into cells of a cardiac lineage. Real-time PCR analysis of cardiac lineage marker genes in Fabry-iPS-derived contracting
cells and mouse ES cell (RF8)-derived contracting cells. Gapdh was used as an internal control.
(C) Transduction of Fabry-iPS cells with a lentiviral vector (LV)/enGFP vector at a multiplicity of infection of 84. Transduced Fabry-iPS cells maintained SSEA-1
expression (upper right panel).
(D) Intracellular α-galactosidase A (α-gal A) activity in LV/α-gal A-transduced Fabry-iPS cells.
(E) Secreted α-gal A activity in LV/α-gal A-transduced Fabry-iPS cells.