Figure 4:Semi-quantitative RT-PCR analysis from centromeric minor satellite RNA and major satellite RNA species.
A. Illustration of the Major and Minor satellite repeat, including the different lengths of the fragment of PCR amplicons
B. RT-PCR products using Minor and Major satellite transcription specific primers to detect Minor and Major satellite RNA transcripts. PCR products are separated on DNA agarose gels with Hprt as input control.