Figure 3:Production of Ang2 and Tie2 in ECs co-cultured with HC- and SSc-MSCs. (a) Ang2 mRNA expression in ECs before and after co-culture with MSCs. SSc- ECs expressed higher levels of Ang2 in each co-culture condition, compared to those observed, when cultured alone. (**=p=0.0002; ***=p<0.0001). (b) Tie2 mRNA expression in ECs before and after co-culture with MSCs. SSc-ECs expressed lower levels of Tie2 in each co-culture condition, compared to those observed, when cultured alone. (**=p=0.0002; ***=p<0.0001). (c) Ang2 ELISA assays. The Ang2 protein production in the supernatants mirrored the results observed by qRT-PCR and western blot analysis (**=p=0.0002; ***=p<0.0001). (d) Western blot analysis of the Ang2 and Tie2 proteins: these results confirmed at protein level the results of qRTPCR analyses. Pictures are representative of all experiments. Tubulin was measured as a loading control for normalization. (*=p=0.008, **=p=0.0002, ***=p=0.0001).