Figure 6: Effects of cmAFSCs and cmADSCs on TGFβ1-induced synthesis of fibronectin in HDF-27 and HDF-73. Fibroblasts were treated with or without TGFβ1 and in combination with either CM of AFSCs or ADSCs for 48 h. (A) mRNA levels of FN1 were measured with qRT-PCR and expressed as fold-change compared to untreated control. (B) Representative immunofluorescence stainings and quantification of the % of cells positive for FN in HDF-27 (upper panel) and HDF-73 (lower panel). White scale bar represents 200 μm. The sign # represents statistically significance towards the untreated control.