Figure 9: Effects of bFGF-neutralizing antibodies on αSMA and collagen synthesis in TGFβ1-stimulated HDF-73. Fibroblasts were treated with or without cmAFSCs in the presence or absence of bFGF-neutralizing antibodies for 48 h. (A) mRNA levels of ACTA2 and COL1A1 were measured with qRT-PCR and expressed as foldchange. (B) Representative immunofluorescence stainings and quantification of the % of cells positive for αSMA and collagen type I. White scale bar represents 200 μm.