Figure 6: Dopaminergic neurons as sole final product at end of cell culture. Using RT-qPCR-TaqMan with specific primers after the 6-month culture, only the boosted expression of the TH gene mRNAs was detected in the re-programmed stem cell-derived dopaminergic neurons (a, red color curve). Doublecortin (Dcx), choline acetyltransferase (Chat), serotonin (SLC6A4), and norepinephrine (SLC6A2) did not present any gene expression. The control group of parallel 6-month cultured non-programmed tail cells did not have any gene expression, including the TH gene (b, green color curve). A summary figure of these data from three independent experiments with the normalized housekeeping gene GAPDH is placed at the top left.