Figure 2: Flow cytometry analysis of peripheral blood. A. Representative profile of peripheral blood with respect to size (FSC) and granularity (SSC). The region defined by the electronic gate R1 excludes debris and contains cells with low granularity, including stem cells. B. Dot plot showing the staining pattern for CD45 and CD34 for particles within the R1 region. The region defined by the electronic gate R3 contains the CD45dim CD34+ stem cells. C. Quandrant analysis of the R3 region showing fluorescence intensity for the stem cell markers CD34 and CD133, where quadrant R9 contains CD34+ CD133+ stem cells. The percentages shown are relative to the number of particles within region R1.