| GENE |
Forward sequence (5’→3)’ |
Reverse sequence (5’→3) |
| β-Actin |
TCTTTGCAGCTCCTTCGTTG |
ACGATGGAGGGGAATACAGC |
| Brachyury |
CTGTGACTGCCTACCAGAATGAGGAG |
GGTCGTTTCTTTCTTTGGCATCAAG |
| Rdh1 |
CATCTGACAGGCTGGAGACA |
AGTCCTCTGTTCCCAACACG |
| Rdh10 |
AAGGCCTTTCTTCCAACGAT |
CAAGGTAAGGGCAAACCAGA |
| Adh1 |
GTTGAGAGCGTTGGAGAAGG |
TCGCTTCGGCTACAAAAGTT |
| Adh3 |
GTGGGAGTAGCTGCTTCAGG |
TGCATCAGATCAAAGGCTTG |
| Adh2 |
AAAGCATCCGAACAATCCTG |
AGGCTTTCTTTGGCTCATCA |
| Raldh1 |
CTCCTGGCGTGGTAAACATT |
CCATGGTGTGCAAACTCAAC |
| Raldh2 |
TTGCAGATGCTGACTTGGAC |
TCTGAGGACCCTGCTCAGTT |
| Raldh3 |
GTGGAGTTCGCCAAGAAGAG |
AGAAGACCGTGGGTTTGATG |
| Crbp1 |
CTTACTGGACCCGAGAGCTG |
GGAAAATGGGGTTGCCTAAT |
| Crbp2 |
GGTGAAATGGGAGAGTGGAA |
GGAAGTCGTCTCAGGCAGTT |
| Crabp1 |
CAGTGTGTGCAGAAGGGAGA |
AGGTTATCTCCTCGGGCTGT |
| Crabp2 |
TAGTCTCCCTGCAGCCTGTT |
TGCTGTTGGTTTTCGTCTTG |
| Nanog |
CAGGTGTTTGAGGGTAGCTC |
CGGTTCATCATGGTACAGTC |
| Oct3/4 |
GGCGTTCTCTTTGGAAAGGT |
CTTCGGGCACTTCAGAAACA |
| Snai1 |
TTGGGCCAACTTCCCAAGCA |
AGAGAAGGCCTTTCCACAGGT |
| Aebp2 |
ATTGGGACATCGGGACAGCATA |
TGGTCCCAGCAACAGTTATAGGC |
| Cdx1 |
AAGGACCTGTTCTGAGTTGC |
TCGTTGAGCCCTCTGAATCT |
| Cyp26a1 |
TGCAGCTCCTGATTGAGCACT |
TCGCAGCACTGGCTGTAGTTT |
| Foxa1 |
AGACATTCAAGCGCAGCTAC |
TGCTGGTTCTGGCGGTAATA |
| Foxn4 |
AACCTGGCTCGCATCGACAA |
TTTCCGGGCGGTCTGAGATGA |
| Gata6 |
AAAACGCCAACCCCGAGAA |
AGTGGTATGAGGCCTTCAGA |
| Otx2 |
TGGAACAAGTGGCCAGTTCA |
AAGTGGACAAGGGGTCAGACA |
| Arid1a |
TCAGCCTGTGTTGAAGCAGA |
TGGCTGAGGTTGAAGGTCAT |
| Neurog3 |
CGGATGACGCCAAACTTACA |
TAGAAGCTGTGGTCCGCTAT |
| Meox1 |
AACCCCGTTTTCCTTCCA |
CTGCTCGTTGAAGATTCGC |
| Id2 |
TCCAAGCTCAAGGAACTGGT |
TGATGCAGGCTGACGATAGT |
| Mist1 |
TGCGCCAGGCCCTAAATTAT |
TCCTCAGTGACACCAAGCAT |
| Hoxa1 |
AGTTGTGGTCCAAGCTATGGC |
ACCATGGGAGTCGAGAGGTTT |
| Hoxa5 |
CGAGCCACAAATCAAGCACACA |
TCGCTCACGGAACTATGATCTCCA |
| Hoxb4 |
AAGTTCACGTGAGCACGGTA |
GCGTCAGGTAGCGATTGTAGT |
| Rarb |
AGCACCGGCATACTGCTCAAT |
TTGTCCTGGCAAACGAAGCA |
|
