Figure 3: The impact of HSPC and BM-StrC CD44 on cell migration: (A,D) wt, CD44v7ko and CD44v6/v7ko HSPC were seeded in the upper part of a Boyden chamber, the lower part contained (A) BSA (control), 20% FCS, HA, FN or Coll IV or (D) BSA, IL6, SDF1, OPN, SCF, GMCSF or bFGF as stimulus. Cells in the lower chamber were counted after 6 h incubation. The mean percent ± SD (triplicates) of migrating HSPC is shown; significant differences between wt, CD44v7ko and CD44v6/v7ko HSPC: s. (B,E) Migration of HSPC towards matrix proteins and cytokines/chemokines in the presence of anti-CD44; the mean percent ± SD (triplicates) of migrating cells is shown; significant migration inhibition by anti-CD44: *. (C,F,G) CFSE-labeled wt and CD44v6/v7ko HSPC were seeded on HA or a monolayer of wt or CD44v6/ v7ko BM-StrC in absence or presence of anti-CD44; migration of individual cells was followed for 16 h. The mean migration of 20 individual cells / 20 min and examples are shown; (C,F) p-values comparing wt and CD44v6/v7ko HSPC migration on HA, wt and CD44v6/v7ko BM-StrC as well as comparing migration on wt versus CD44v6/ v7ko BM-StrC are indicated, significant inhibition by anti-CD44: *. HSPC migration is significantly influenced by HSPC CD44v6 and BM-StrC CD44v7.