Figure 5: The impact of CD44v6 and CD44v7 on cell cycle progression: (A,B) Flow-cytometry analysis of expression of Wnt- and TGFβ-regulated genes in CD44wt and CD44v6/v7ko HSPC cultured on BSA, HA, wt or CD44v6/v7ko BM-StrC; percent stained HSPC (mean ± SD, triplicates) and examples; significant differences by culture condition: +, significant differences between CD44wt and CD44v6/v7ko HSPC: s. TGFβ upregulation in CD44v6/v7ko BM-StrC (Suppl.Fig.4) was not accompanied by altered expression of TGFβ-regulated genes with the exception of a slight reduction in CD117+ cells. In addition, Wnt-regulated gene expression varied, which points towards a multifactorial process and significant contributions of CD44-independent components.