Gene Fold change after differentiation p-value Nanog -1.23 0.03 Sox2 -1.04 0.31 Hnf4(alpha) 1.05 0.03 Gata4 1.63 1.73E-04 Gata6 -1.34 2.35E-04 Nkx2.2 1.05 0.62 Sox9 2.64 6.85E-05 Pax6 -1.7 7.00E-04 NeuroD2 1.09 0.19 MafB -2.79 1.39E-05 Insulin2 1.28 0.01 BMPR1A -1.38 3.16E-04 BMPR1B -1.48 2.59E-05 BMPR2 -1.14 0.03 CD34 7.83 1.49E-06 CD44 2.46 9.32E-06 Lin54 2.41 1.14E-06 Lin7b 1.16 9.56E-03 Lin7c 1.15 4.27E-05 Lin9 3.11 1.48E-05 Lynx1(Sca-1) 1.14 0.02 Ly6a 1.29 0.04 Ly6c1 1.39 0.04 Ly6c2 1.39 0.03 Ly6e -1.04 0.01 Ly6g6f -1.12 0.04 Thy1 -1.61 1.59E-03 Alcam 4.82 1.02E-05 Cdh2 (N-Cadherin) 1.66 2.15E-06 Uchl1 1.73 2.27E-03 Fn1 (Fibronectin) 1.1 0.04 Itga1 -2.35 1.78E-03 Gnl3 1.3 6.14E-03 Pdgfra -1.92 7.89E-05 Vcam1 1.06 0.02 Vim (Vimentin) 1.11 2.38E-04

Table 1: Pancreatic and mesenchymal markers in differentiated cells. The first two genes correspond to undifferentiated markers. The genes highlighted in grey correspond to pancreatic markers and the rest to mesenchymal or hematopoietic markers. Data were analyzed using the Ambion® WT Expression Kit For Affymetrix® GeneChip® and filters were applied to pancreatic genes showing a fold change ≥ 1 with an adjusted p-value <0.05 for each experiment condition (positive fold change indicates gene upregulation and negative fold change indicates gene downregulation).