Figure 1: Diagram showing differentiation process of mESCs into either motor neurons or oligodendrocytes and schematic of two-compartmentalized microfluidic platform. (A) Diagram showing motor neuron (MNs) and oligodendrocytes induction from mESCs. (B) For MN differentiation, EBs were induced from mESCs by adding 2-/6+RA and Pur for 8 days and followed treatment with GDNF and BDNF for 5 to 7 days. OPCs were derived from EBs in the presence of FGF2 and PDGF for 12 to 14 days. (C) Schematic overview of MNs and oligodendrocytes co-culture system in the two compartmentalized PDMS platform. Soma and axons of MNs are separated in this microfluidic platform. Only the axons pass through the microchannels and reach the OPCs compartment.