After 12-14 days of GDNF and BDNF treatment, the cells were stained with anti-O1 (green, A) and anti-GFAP (red, B). Most of the cells are O1+, indicating mESCs are successfully induced to differentiate into oligodendrocytes. Oligodendrogenesis is further confirmed by immunostaining with oligodendrocyte lineage markers like anti-A2B5 (D), anti-NG2 (E), anti-O4 (F), anti-O1 (G), anti-MBP (H) and anti-CNPase (I). All cells are nuclear counterstained with Hoechst (blue). Scale bar: 100 μm.
