Figure 2: Effect of donor age and passage number on cell morphology and proliferative capacity in cultured ASCs. (A) Photomicrographs show the morphology of cultured ASCs at passage 4 prepared from donors aged 5 months (5 M), 1 year (1 Y), 60 years (60 Y), and 82 years (82Y). Scale bar: 200 μm. (B) Proliferative capacity of cultured ASCs from each individual during the passaging of cells is shown. S indicates cellular senescence. Arrows indicate periods at which conditioned media were collected for analysis.