Figure 6: Effect of donor age and passage number on the concentration of angiogenic factors in ASC-CM. Conditioned media were collected from cultured ASCs at passages 2, 4, and 6 from donors of different ages. The expression levels of vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF) in ASC-CM were measured with ELISA. (A) The concentrations of VEGF-A and HGF in each sample are shown. Data shown are the mean ± SD of results from triplicate dishes. nd indicates not detectable. (B) The samples were divided into two groups: donor age >26 years and donor age <11 years. The levels of VEGF-A were compared between the two groups at each passage. Data shown are the mean ± SD. *p < 0.05 (Mann-Whitney U test).