hiPSC clones were generated using episomal (Epi) plasmids or Sendai Virus (SeV) to express the reprogramming factors. (A) Morphology of a representative emerging hiPSC colony generated by episomal plasmids and (B) by Sendai viruses. Images in (a) lower (1000X) and (b) higher (2000X) magnifications are shown to represent the defined boundaries and the increased nuclear to cytoplasmic ratio of the emerging colonies. (C) Morphology and immunofluorescence analysis of pluripotency markers in an established Epi-hiPSC line and (D) in an established SeV-hiPSC line. (E) Real time PCR analysis of pluripotency markers in Epi-hiPSC lines and (F) in SeV-hiPSC lines (n=2).The fold changes were calculated relative to the expression levels in hESCs.
