Figure 1: Glioblastoma. (A) Circumscribed necroses develop in a zone poor of small vessels (CD34 immunostaining, DAB), from (B) hypercellular areas (H&E), leaving (C) pseudopalisading as their residue (H&E). (D) Hypercellular areas are poor in GFAP expression (DAB), with (E) high Nestin expression (DAB), (F) high Ki-67/MIB-1 labeling index (DAB), (G) high SOX2 expression (DAB) and (H) high REST expression (DAB). Scale bar: 25 μm.