Enzyme Source Optimum pH Range ECM Substrates Activator(s) Common Inhibitors Collagenase type I and type II Clostridium histolyticum 6.3-8.5 Collagen I, II, III and IV Ca2+, Zn2+ EDTA, EGTA Blood Plasma/Serum Dispase Paenibacilluspolymyxa 5.9-7.0 Fibronectin, Collagen IV Collagen I (small amounts) Ca2+, Mg2+ EDTA, EGTA Thermolysin Geobacillus stearothermophilus,        Bacillus thermoproteolyticus 5.0-8.5 Non-specific Cell adhesion proteins Ca2+, Zn2+ EDTA, EGTA Trypsin Susdomesticus (porcine) Bostaurus (bovine) 7.0-9.0 Non-specific Cell adhesion proteins Ca2+ EDTA, EGTA Blood Plasma/Serum Clostripain Clostridium histolyticum 7.4-7.8 Non-specific Cell Adhesion protein Ca2+ EDTA, EGTA

*insufficient data pertaining specifically to CHNP

Table 1: Summary of tissue dissociation enzymes.