| Enzyme |
Unit |
Definition |
Conversions |
Trypsin and
Trypsin-like
proteases
|
BAEE Units (S & T units)
|
One unit catalyzes a change in absorbance at 253 nm of 0.001 per minute at 25°C, pH 7.6.
Substrate: N-benzoyl-L-arginine ethyl ester
(BAEE)
|
1 TAME Unit = 57.5 BAEE units
1 TAME Unit = 19.2 USP units
1U = 4.69 TAME Units
1 U = ~ 270 BAEE Units
1U = 90 USP Units
1 USP unit =3 BAEE units |
Neutral Protease Units
(NPU) |
An increase of 1 fluorescent unit per minute at 35°CBSAfromconjugatethecleava. |
USP Units
(NF Units) |
1 USP-unit catalyzes a change in absorbance at 253 nm of 0.003 per minute at 25 °C, pH 7.6
Substrate: N-benzoyl-L-arginine ethyl ester
(BAEE) |
| TAME Units |
One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 30°C, pH 8.2 |
| DMC-U units |
One DMC-U catalyzes the cleavage of 1 μmole of peptide bonds from dimethyl casein (DMC), at 25°C, pH 7.0 |
International Units
(U) |
One international unit catalyzes the hydrolysis of 1 μmole Nα-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C, pH 8.0
|
