Buffer Composition pH range Lactated Ringer’s Solution (LR) 130 mM Na+ 6.0-7.5 109 mMCl- 28 mM lactate 1.5 mM Ca2+ 4 mM K+ Hank’s Balanced Salt Solution (HBSS) with Calcium added     0.137 M NaCl 7.0-7.4       5.4 mMKCl 0.25 mM Na2HPO4 0.1g glucose 0.44 mM KH2PO4 1.3 mM CaCl2 1.0 mM MgSO4 4.2 mM NaHCO3 Dulbecco’s Phosphate Buffered Saline (PBS or DPBS)   8 mM Na2HPO4 7.0-7.2 135mM NaCl 1 mM CaCl2 3 mMKCl 0.5 mM MgCl2 1.5 mM KH2PO4

Table 5: Common buffers used for tissue dissociation.